To trace particle movement:

  1. Open an image that contains time-lapse data in the Image window.

  2. From the main softWoRx window, choose View | Trails Movie.

  3. In the Trails Movie Input field, enter the Image window number.

  4. Select a region of interest. Then select which wavelengths to include in the movie.

  5. In the Window field, enter the length of the trace history (e.g., for a value of 5, the trace includes the previous 4 time frames and the current time frame).

  6. To emphasize the display of the current point, enter a value greater than 1.0 in the Enhancement Factor field (typical values for this field are 1.0 - 2.0).

  7. Note The displayed intensity value of each point is a weighted average of the  corresponding points in the previous time frames. The previous points each  have a weight of 1.0. The Enhancement factor is assigned as the weight for the intensity of the current value.

  8. Click Do It to create the trails display.

  9. To create a Trails movie, choose File | Save As Movie on the Image window and save the movie.