Click the Settings 
icon in the Resolve3D main menu to open the Settings menu. Then activate
the Autofocus tab and click on
the Calibrate UltimateFocus
for plate scanning button. Follow the instructions in the UltimateFocus
Plate Calibration window to complete the calibration process as in the
steps that follow.
Place a multi-well plate on the microtiter stage. (See Loading a Plate for details.)
From the Resolve3D main menu, select the Microtiter Plate icon. A representation of the microtiter plate is displayed in the Plate View window.
Select the Visit Wells tool and then select a well with visible sample material, preferably at the bottom of the well.
Manually lower the objective turret to its minimum position.
Click the Home Stage Z button at the bottom of the Ultimate Focus Plate Calibration window to “zero” the microtiter stage Z coordinate.
Using the eyepieces and the microscope’s manual focus controls, raise the objective to focus on the sample material at the bottom of the well.
Click the Calibrate Focus button at the bottom of the Ultimate Focus Plate Calibration window to start the calibration process.
Note This procedure may take a few minutes to complete.