icon.Maximum signal-to-noise ratio will be achieved when fluorophores switch on and then off again within a single image frame. Higher TIRF laser power density leads to more efficient photoconversion, allowing for effective switching with shorter exposure times. The Beam Concentrator can be used to increase the TIRF laser power density reaching the sample.
Note: Using the Beam Concentrator limits your usable field of view to 512x512 pixels. |
To use the Beam Concentrator:
1. From the Resolve3D window, click the
Settings icon.
2. On the Resolve3D Settings window, click the Lasers tab
3. Click the TIRF Illumination Settings button near the bottom of the window. The TIRF Illumination Settings window is displayed.
4. Activate the Use TIRF Beam Concentrator check box
5. Proceed to set up TIRF imaging as described in the “Imaging Using TIRF” section in Chapter 8 of the DeltaVision Imaging System User’s Manual.
Calibrating the Camera for Localization