Selecting By Frame (default) displays
only the positions found in the active image frame. The plotted positions
update as the user scrolls through time.To use the Localization Results Viewer:
1. From the softWoRx main menu, select Measure | Localization Results Viewer. The Localization Results Viewer is displayed.
2. Drag and drop an image window number, or enter an image window number, into the Window field.
3. Click the Localization Data button to browse to and select the localization file that corresponds to the Window field input. Alternatively, you can click and drag a localization file from the data folder into this field. This file must be a DeltaVision localization file (*_LOC.txt or *_LDC.txt). The tool will plot the localization points from the localization file and overlay them on the input image window.
4. Select a Region of Interest (ROI) to display the detected fluorophore positions within the region. Multiple ROIs may be chosen using the Create Rectangular ROI Polygon, Create ROI Polygon, or Create Freehand ROI Polygon tools. ROIs may be closed with the Close Polygon tool. ROIs may be deleted by selecting them with the Select ROI Polygon tool then clicking the Delete Selected Polygon button.
5. Choose whether to shift the detected fluorophore positions points by half a pixel. By default the detected fluorophore positions are shifted by half a pixel to center the positions in the middle of the image pixel. If the Shift points by half sample check box is deselected, the positions are plotted on the lower-left corner of the image pixel.
6. Activate the Show points on image check box to display the point positions in the Image window.
7. Select how to display the detected fluorophore positions from the Localized point view style drop-down menu.
Selecting By Frame (default) displays
only the positions found in the active image frame. The plotted positions
update as the user scrolls through time.
Selecting Cumulative displays
all the positions that were detected from the beginning of the data set
up to and including the active image frame. The plotted positions update
as the user scrolls through time.
Selecting All Points displays
every detected fluorophore position in the input Localization Data file.
8. Select the Localized point display color from the drop-down menu. The default color is Red, but other marker colors may be used according to user preference.
9. Select the Localized point marker style from the drop-down menu. The default shape is Dot, but other markers may be selected according to user preference.
10. Scroll through the image window and assess whether the plotted positions match the expected peaks from the raw data in the image window.
The Localization Results Viewer
The Localization Precision Histogram